Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ZNRF2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 192 and 242 of human zinc and ring finger 2 using the numbering given in entry NP_667339.1 (GeneID 223082).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNRF2 Antibody

Western Blot: ZNRF2 Antibody [NBP1-28715]

Western Blot: ZNRF2 Antibody [NBP1-28715]

Western Blot: ZNRF2 Antibody [NBP1-28715] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ZNRF2 antibody NBP1-28715 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. ZNRF2 was also immunoprecipitated by rabbit anti-ZNRF2 antibody NBP1-28697, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for ZNRF2 Antibody

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZNRF2

Zinc- and RING- finger 2 (ZNRF2) is suggested to play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Alternate Names

EC 6.3.2.-, Protein Ells2, RING finger protein 202, RNF202E3 ubiquitin-protein ligase ZNRF2, zinc and ring finger 2, zinc finger/RING finger 2, Zinc/RING finger protein 2

Entrez Gene IDs

223082 (Human)

Gene Symbol

ZNRF2

UniProt

NP_667339.1 (Human)

Additional ZNRF2 Products

Product Documents for ZNRF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNRF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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