ZP3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94555

Novus Biologicals, part of Bio-Techne
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NBP2-94555-0.02ml
NBP2-94555-0.1ml
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ZP3 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 170-390 of human ZP3 (NP_001103824.1). LTFSLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPTPDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFANDSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCCNKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGPLIFLDRRGDHEVEQWALPSDTSVVLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZP3 Antibody - BSA Free

Western Blot: ZP3 AntibodyBSA Free [NBP2-94555]

Western Blot: ZP3 AntibodyBSA Free [NBP2-94555]

Western Blot: ZP3 Antibody [NBP2-94555] - Analysis of extracts of SKOV3 cells, using ZP3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: ZP3 Antibody - BSA Free [NBP2-94555]

Immunocytochemistry/ Immunofluorescence: ZP3 Antibody - BSA Free [NBP2-94555]

Immunocytochemistry/Immunofluorescence: ZP3 Antibody [NBP2-94555] - Analysis of rat oophoroma cells using ZP3 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ZP3 Antibody - BSA Free [NBP2-94555]

Immunocytochemistry/ Immunofluorescence: ZP3 Antibody - BSA Free [NBP2-94555]

Immunocytochemistry/Immunofluorescence: ZP3 Antibody [NBP2-94555] - Analysis of rat oophoroma cells using ZP3 at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for ZP3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZP3

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq]

Alternate Names

zona pellucida glycoprotein 3 (sperm receptor)

Gene Symbol

ZP3

Additional ZP3 Products

Product Documents for ZP3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ZP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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