ZW10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38644

Novus Biologicals, part of Bio-Techne
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NBP2-38644-25ul
NBP2-38644
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZW10 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZW10 Antibody

Western Blot: ZW10 Antibody [NBP2-38644]

Western Blot: ZW10 Antibody [NBP2-38644]

Western Blot: ZW10 Antibody [NBP2-38644] - Analysis using Anti-ZW10 antibody NBP2-38644 (A) shows similar pattern to independent antibody NBP2-38751 (B).
Immunocytochemistry/ Immunofluorescence: ZW10 Antibody [NBP2-38644]

Immunocytochemistry/ Immunofluorescence: ZW10 Antibody [NBP2-38644]

Immunocytochemistry/Immunofluorescence: ZW10 Antibody [NBP2-38644] - Staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ZW10 Antibody [NBP2-38644]

Immunohistochemistry-Paraffin: ZW10 Antibody [NBP2-38644]

Immunohistochemistry-Paraffin: ZW10 Antibody [NBP2-38644] - Staining of human stomach, upper shows moderate cytoplasmic positivity in glandular cells.

Applications for ZW10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZW10

ZW10 encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase.

Alternate Names

E3 ubiquitin-protein ligase ZSWIM2, EC 6.3.2.-, MEKK1-related protein X, MEX, MGC33890, Zinc finger SWIM domain-containing protein 2, zinc finger, SWIM domain containing 2, zinc finger, SWIM-type containing 2, ZZZ2ZZ-type zinc finger-containing protein 2

Gene Symbol

ZW10

UniProt

O43264 (Human)

Additional ZW10 Products

Product Documents for ZW10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZW10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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