ZWINT Antibody (6D3)

Novus Biologicals, part of Bio-Techne | Catalog # H00011130-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6D3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZWINT Antibodies »

Product Specifications

Immunogen

ZWINT (AAH20979, 1 a.a. ~ 277 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDGVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP

Reactivity Notes

Human. Other species not tested.

Specificity

ZWINT - ZW10 interactor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ZWINT Antibody (6D3)

Western Blot: ZWINT Antibody (6D3) [H00011130-M01]

Western Blot: ZWINT Antibody (6D3) [H00011130-M01]

Western Blot: ZWINT Antibody (6D3) [H00011130-M01] - ZWINT monoclonal antibody (M01), clone 6D3 Analysis of ZWINT expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: ZWINT Antibody (6D3) [H00011130-M01]

Immunocytochemistry/ Immunofluorescence: ZWINT Antibody (6D3) [H00011130-M01]

Immunocytochemistry/Immunofluorescence: ZWINT Antibody (6D3) [H00011130-M01] - Analysis of monoclonal antibody to ZWINT on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: ZWINT Antibody (6D3) [H00011130-M01] - Detection limit for recombinant GST tagged ZWINT is approximately 0.03ng/ml as a capture antibody.

Applications for ZWINT Antibody (6D3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZWINT

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

C20orf164, chromosome 20 open reading frame 164, dJ337O18.7, zinc finger SWIM domain-containing protein 3, zinc finger, SWIM domain containing 3, zinc finger, SWIM-type containing 3

Entrez Gene IDs

11130 (Human)

Gene Symbol

ZWINT

OMIM

609177 (Human)

UniProt

AAH20979 (Human)

Additional ZWINT Products

Product Documents for ZWINT Antibody (6D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZWINT Antibody (6D3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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