ZWINT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2364

Novus Biologicals, part of Bio-Techne
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NB100-2364
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ZWINT Antibodies »

Product Summary for ZWINT Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 225 and the C-terminus (residue 277) of human ZW10 Interactor-1 using the numbering given in entry NP_008988.2 (GeneID 11130).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZWINT Antibody

Western Blot: ZWINT Antibody [NB100-2364]

Western Blot: ZWINT Antibody [NB100-2364]

Western Blot: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 on HeLa whole cell lysate using NB100-2364. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody NB100-2363.
Immunocytochemistry/ Immunofluorescence: ZWINT Antibody [NB100-2364]

Immunocytochemistry/ Immunofluorescence: ZWINT Antibody [NB100-2364]

Immunocytochemistry/Immunofluorescence: ZWINT Antibody [NB100-2364] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-ZWINT-1 used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.

Applications for ZWINT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
ICC/IF Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZWINT

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

C20orf164, chromosome 20 open reading frame 164, dJ337O18.7, zinc finger SWIM domain-containing protein 3, zinc finger, SWIM domain containing 3, zinc finger, SWIM-type containing 3

Entrez Gene IDs

11130 (Human)

Gene Symbol

ZWINT

UniProt

O95229 (Human)

Additional ZWINT Products

Product Documents for ZWINT Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ZWINT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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