Zyxin Antibody (2C10-4A7)

Novus Biologicals, part of Bio-Techne | Catalog # H00007791-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C10-4A7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Zyxin Antibodies »

Product Specifications

Immunogen

ZYX (AAH08743, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAPRPSPAISVSVSAPAFYAPQEKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYECEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT

Specificity

ZYX - zyxin

Marker

Focal Adhesion Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Zyxin Antibody (2C10-4A7)

Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01]

Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01]

Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7.Lane 1: ZYX transfected lysate(61.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (2C10-4A7) [H00007791-M01]

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (2C10-4A7) [H00007791-M01]

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of monoclonal antibody to ZYX on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01]

Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01]

Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi ( Cat # H00007791-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZYX monoclonal antibody (M01), clone 2C10-4A7 (Cat # H00007791-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
View 2 more images

Applications for Zyxin Antibody (2C10-4A7)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA, RNAi Validation and IF.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Zyxin

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Alternate Names

Zyx, Zyxin 2

Entrez Gene IDs

7791 (Human)

Gene Symbol

ZYX

OMIM

602002 (Human)

UniProt

AAH08743 (Human)

Additional Zyxin Products

Product Documents for Zyxin Antibody (2C10-4A7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Zyxin Antibody (2C10-4A7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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