Zyxin Antibody (CL2502)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36768

Novus Biologicals, part of Bio-Techne
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NBP2-36768
NBP2-36768-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2502

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Zyxin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:PAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNE

Epitope

APGPLTLKEVEELEQ

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (84%), Rat (85%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Zyxin Antibody (CL2502)

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in U251 cell line showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768]

Immunohistochemistry-Paraffin: Zyxin Antibody (CL2502) [NBP2-36768] - Staining of human kidney shows moderate to strong cytoplasmic and membranous positivity in cells in glomeruli and in tubules.
Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/ Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768]

Immunocytochemistry/Immunofluorescence: Zyxin Antibody (CL2502) [NBP2-36768] - Staining in HeLa cell line showing distinct focal adhesion staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
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Applications for Zyxin Antibody (CL2502)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Zyxin

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Alternate Names

Zyx, Zyxin 2

Gene Symbol

ZYX

UniProt

Q15942

Additional Zyxin Products

Product Documents for Zyxin Antibody (CL2502)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Zyxin Antibody (CL2502)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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