Zyxin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46135

Novus Biologicals, part of Bio-Techne
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NBP1-46135
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Zyxin Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QQREKPRVQEKQH corresponding to internal region according to NP_003452.1.

Epitope

internal region

Specificity

Reported variants represent identical protein: NP_003452.1, NP_001010972.1

Marker

Focal Adhesion Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Zyxin Antibody

Western Blot: Zyxin Antibody [NBP1-46135]

Western Blot: Zyxin Antibody [NBP1-46135]

Western Blot: Zyxin Antibody [NBP1-46135] - (0.1ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Zyxin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 85 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 61.3 kDa band according to NP_003452.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Zyxin

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Alternate Names

Zyx, Zyxin 2

Entrez Gene IDs

7791 (Human)

Gene Symbol

ZYX

UniProt

NP_003452.1 (Human)

Additional Zyxin Products

Product Documents for Zyxin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Zyxin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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