Donor Horse Serum

Donor Horse Serum (DHS) is derived from healthy, live animals greater than 12 months of age. The donor animals receive regular veterinary inspection and care, and are kept in carefully managed and strictly segregated herds. The donor animals are all gelded draft horses in order to limit lot-to-lot hormone level variations A variety of cell types can be successfully propagated in DHS. It is frequently used in combination with bovine sera or independently to supplement media for mammalian cell culture. DHS is often utilized in bovine virus research applications since it does not contain bovine antibodies. It is also used as a supplement in some bacteriological media. Donor Horse Serum, Heat Inactivated is treated by heat inactivation where serum is exposed to a temperature of 56 °C for 30 minutes under controlled conditions.

Serum products should be stored and handled correctly to assure long-term stability and to preserve growth performance consistency throughout its shelf-life. In addition, heat inactivation of serum is frequently desired to inactivate complement within the serum. Below are protocol links for serum storage/handling and heat inactivation.

Protocol for Fetal Bovine Serum Storage, Thawing, and Freezing.
Protocol for Heat Inactivation of Serum Products.