TcBuster™-M GMP Transposase mRNA
R&D Systems, part of Bio-Techne | Catalog # TCB-001.1-GMP
Key Product Details
| Features: | TcBuster-M Transposase mRNA encodes a hyperactive version of the TcBuster transposase. As a component of the TcBuster non-viral gene delivery system, this product requires a compatible TcBuster DNA transposon to function. This two-component system can be introduced, by electroporation, into a wide variety of cell types to enable stable gene transfer. |
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Mechanistic Diagram of the TcBuster Non-Viral Gene Editing System. The TcBuster system components are introduced into cells via electroporation (1). The TcBuster-M transposase mRNA is then translated into the transposase enzyme (2), which binds to the inverted terminal repeats (ITRs) on the DNA transposon (3). The transposase enzyme excises the genes of interest (GOI) from the transposon (4) and inserts them into the host genome (5). The GOI mRNA is transcribed from the host genome (6), and the protein is stably expressed in the edited cells (7). |
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