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Lightning-Link (R) APC-Cy7 Antibody Labeling Kit

Novus Biologicals, part of Bio-Techne | Catalog # 765-0010

Novus Biologicals, part of Bio-Techne
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765-0010

Key Product Details

Applications

Flow Cytometry

Kit Type

Antibody Labeling Kit

Conjugate

Allophycocyanin/Cy7

Product Summary for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit

Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates.

FeaturesBenefits
Quick and easy to useSave time, no special knowledge required
No separation steps100% recovery - no antibody/protein loss
Can be used in a wide range of applicationsFlexible
Freeze driedShips at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)Easy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 referencesSuccessfully used in many fields of research


APC/Cy7 is a tandem conjugate. The APC is excited at 652nm and functions as an energy donor for the Cy7. Energy is transferred from the APC to the Cy7 via FRET (Fluorescence Resonance Energy Transfer). The Cy7 emits the energy received from the APC in the form of long wavelength light at 790nm

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit is supplied as 3 vials.

Scientific Data Images for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit

Flow Cytometry: Lightning-Link APC-Cy7 Antibody Labeling Kit [765-0010]

Kit Contents for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit

  • 1 or 3 glass vial(s) of Lightning-Link mix
  • 1 vial of LL-Modifier reagent
  • 1 vial of LL-Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) APC-Cy7

Lightning-Link is a one step antibody labeling kit, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.Label - APC/Cy7 is a tandem conjugate. The APC is excited at 633nm and functions as an energy donor for the Cy7. Energy is transferred from the APC to the Cy7 via FRET (Fluorescence Resonance Energy Transfer). The Cy7 emits the energy received from the APC in the form of long wavelength light at 767nm.Features:- 30 seconds hands-on time- No separation steps- No losses- Scalable technology

Additional Lightning-Link (R) APC-Cy7 Products

Product Documents for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lightning-Link (R) APC-Cy7 Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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