Lightning-Link (R) PerCP Antibody Labeling Kit

Novus Biologicals, part of Bio-Techne | Catalog # 718-0030

Novus Biologicals, part of Bio-Techne
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Key Product Details

Applications

Flow Cytometry

Kit Type

Antibody Labeling Kit

Conjugate

PerCP

Product Summary for Lightning-Link (R) PerCP Antibody Labeling Kit

Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

Our PerCP antibody labeling kit enables the direct conjugation of PerCP to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link PerCP and incubates for 3 hours.

FeaturesBenefits
Quick and easy to useSave time, no special knowledge required
No separation steps100% recovery - no antibody/protein loss
Can be used in a wide range of applicationsFlexible
Freeze driedShips at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)Easy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 referencesSuccessfully used in many fields of research


PerCP is a small 35kDa phycobiliprotein isolated from red algae. It has a strong absorption peak at 484nm and a large stokes shift (194nm) with its maximum emission peak at 678nm. PerCP is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit is supplied with 3 vials, each suitable for labeling up to 10 ug of antibody.

Scientific Data Images for Lightning-Link (R) PerCP Antibody Labeling Kit

Flow Cytometry: Lightning-Link (R) PerCP Antibody Labeling Kit [718-0030]

Flow Cytometry: Lightning-Link (R) PerCP Antibody Labeling Kit [718-0030]

Flow Cytometry: Lightning-Link PerCP Antibody Labeling Kit [718-0030] - Mouse anti-human CD3 was conjugated with PerCP using a Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).

Kit Contents for Lightning-Link (R) PerCP Antibody Labeling Kit

  • 1 or 3 glass vial(s) of Lightning-Link mix
  • 1 vial of LL-Modifier reagent
  • 1 vial of LL-Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) PerCP

Easy PerCP labeling of your antibody with 30 seconds hands-on time. PerCP is a small 35kDa phycobiliprotein isolated from red algae. It has a strong absorption peak at 482nm and a secondary absorption peak at 442nm. PerCP has a large stokes shift (195nm) with its maximum emission peak at 677nm. PerCP The protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting.

The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

Additional Lightning-Link (R) PerCP Products

Product Documents for Lightning-Link (R) PerCP Antibody Labeling Kit

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lightning-Link (R) PerCP Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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