Lightning-Link (R) Rapid DyLight 550 Antibody Labeling Kit
Novus Biologicals, part of Bio-Techne | Catalog # 323-0030
Key Product Details
Applications
Kit Type
Conjugate
Product Summary for Lightning-Link (R) Rapid DyLight 550 Antibody Labeling Kit
The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.
The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates (for more details please watch the video below).
Features | Benefits |
Quick and easy to use | Save time, no special knowledge required |
No separation steps | 100% recovery - no antibody/protein loss |
Can be used in a wide range of applications | Flexible |
Freeze dried | Ships at ambient temperature, long shelf-life |
Fully scalable (10 ug to 1 g or more) | Easy transfer from R&D to manufacturing |
Stringently QC tested | Consistent high quality, excellent batch-to-batch reproducibility |
Large number of labels available | Experimental flexibility |
Reliable: nearly 300 references | Successfully used in many fields of research |
DyLight 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield.
DyLight dyes are extremely bright and are highly water soluble meaning that a high dye-protein ratio can be achieved without precipitation of the conjugates. Furthermore DyLight dyes retain their fluorescence over a pH range of 4-9.
Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay
Product Specifications
Application Notes
Scientific Data Images for Lightning-Link (R) Rapid DyLight 550 Antibody Labeling Kit
Flow Cytometry: Lightning-Link (R) Rapid DyLight 550 Antibody Labeling Kit [323-0030]
Flow Cytometry: Lightning-Link Rapid DyLight 550 Antibody Labeling Kit [323-0030] - Mouse anti-human CD3 was conjugated with Dylight® 550 using Lightning-Link ® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).Kit Contents for Lightning-Link (R) Rapid DyLight 550 Antibody Labeling Kit
- 1 or 3 glass vial(s) of Lightning-Link Rapid mix
- 1 vial of LL-Rapid Modifier reagent
- 1 vial of LL-Rapid Quencher reagent
Formulation, Preparation, and Storage
Concentration
Shipping
Storage
Background: Lightning-Link (R) Rapid DyLight 550
- Allows direct labeling of antibodies, proteins, peptides or other biomolecules. Procedure is completed in seconds, and used within minutes (less than 20 minutes). Process generates conjugates identical, or better than, traditional conjugation procedures. 100 percent recovery.
For more information please check out these useful links!
http://www.novusbio.com/support/support-by-application/antibody-conjugation/illustrated-assay.html">Antibody Labeling Guide
http://www.novusbio.com/support/support-by-application/antibody-purification/illustrated-assay.html">Purifaction Antibody Guide
https://www.facebook.com/video/video.php?v=121668471181129">Lightning-Link® Demonstration Video
http://images.novusbio.com/design/fluorescent_poster.pdf">Fluorescent Labels Poster
Additional Lightning-Link (R) Rapid DyLight 550 Products
Product Documents for Lightning-Link (R) Rapid DyLight 550 Antibody Labeling Kit
Product Specific Notices for Lightning-Link (R) Rapid DyLight 550 Antibody Labeling Kit
This product is manufactured by Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.