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Lightning-Link (R) Rapid DyLight 633 Antibody Labeling Kit

Novus Biologicals, part of Bio-Techne | Catalog # 325-0010

Novus Biologicals, part of Bio-Techne
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325-0010

Key Product Details

Applications

Flow Cytometry

Kit Type

Antibody Labeling Kit

Conjugate

DyLight 633

Product Summary for Lightning-Link (R) Rapid DyLight 633 Antibody Labeling Kit

Lightning-Link Rapid is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.

The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates (for more details please watch the video below).

FeaturesBenefits
Quick and easy to useSave time, no special knowledge required
No separation steps100% recovery - no antibody/protein loss
Can be used in a wide range of applicationsFlexible
Freeze driedShips at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)Easy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 referencesSuccessfully used in many fields of research


DyLight 633 provides red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 628nm, high fluorescence at 660nm (extinction coefficient 1.7 x105 cm-1M-1) and high quantum yield.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%.

Scientific Data Images for Lightning-Link (R) Rapid DyLight 633 Antibody Labeling Kit

Flow Cytometry: Lightning-Link (R) Rapid DyLight 633 Antibody Labeling Kit [325-0010]

Flow Cytometry: Lightning-Link (R) Rapid DyLight 633 Antibody Labeling Kit [325-0010]

Flow Cytometry: Lightning-Link Rapid DyLight 633 Antibody Labeling Kit [325-0010] - Mouse anti-human CD3 was conjugated with Dylight® 633 using an Innova Biosciences Rapid Lightning-Link® kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).

Kit Contents for Lightning-Link (R) Rapid DyLight 633 Antibody Labeling Kit

  • 1 or 3 glass vial(s) of Lightning-Link Rapid mix
  • 1 vial of LL-Rapid Modifier reagent
  • 1 vial of LL-Rapid Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) Rapid DyLight 633

Lightning-Link® Rapid Antibody Labeling kits

Additional Lightning-Link (R) Rapid DyLight 633 Products

Product Documents for Lightning-Link (R) Rapid DyLight 633 Antibody Labeling Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lightning-Link (R) Rapid DyLight 633 Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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