Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit

Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (See protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes).

Our Lightning-Link Rapid technology can be used to label small quantities of antibody with 100% recovery. The researcher simply pipettes the antibody or other biomolecule into a vial of lyophilized mixture containing the label of interest and incubates the reaction for only 15 minutes (for more details please watch the video below). As there are no desalting or dialysis steps that commonly interrupt traditional protein labeling procedures.

Features	Benefits
Quick and easy to use	Save time, no special knowledge required
No separation steps	100% recovery - no antibody/protein loss
Can be used in a wide range of applications	Flexible
Freeze dried	Ships at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)	Easy transfer from R&D to manufacturing
Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available	Experimental flexibility
Reliable: nearly 300 references	Successfully used in many fields of research

The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA. Our biotinylation kits are optimised to create high quality conjugates and minimise assay background.

Lightning-Link Biotin Has been optimized for two separate applications: Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used. Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay