What functional groups do I need on my protein?

Lightning-Link requires amine groups on the molecule to be labeled. Most proteins have lysine and/or alpha-amino groups. All antibodies will have multiple amine functions.

Do I need to purify the conjugate?

No. The chemicals used in Lightning-Link are deactivated by the quencher, and the by-products are benign and do not need to be removed.

Can non-antibody molecules be labeled?

Yes. While labeling of antibodies is a major application, the only requirement is that the protein to be labeled has amine functionality.

I was wondering if you could please let us know how this product differs from the Innova Biosciences product: 708-0030 (has same part #)

Novus distributes products for Innova in the US, so 708-0030 is the same Lightning-Link® Streptavidin Labeling Kit that is also sold by Innova Biosciences.

Lightning-Link (R) Streptavidin Antibody Labeling Kit

Name: Lightning-Link (R) Streptavidin Antibody Labeling Kit
Brand: Novus Biologicals
SKU: 708-0030
Rating: 4 (1 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # 708-0030

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Product Datasheet / COA / SDS / Protocols & Manuals

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Product Summary
Product Specifications
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Kit Type

Antibody Labeling Kit

Conjugate

Streptavidin

Product Summary for Lightning-Link (R) Streptavidin Antibody Labeling Kit

Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

Our streptavidin antibody labeling kit enables the direct conjugation of streptavidin to any biomolecule with an available amine group. The researcher simply pipettes their antibody or other biomolecule into the vial of Lightning-Link Streptavidin and incubates for 3 hours.

Features	Benefits
Quick and easy to use	Save time, no special knowledge required
No separation steps	100% recovery - no antibody/protein loss
Can be used in a wide range of applications	Flexible
Freeze dried	Ships at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)	Easy transfer from R&D to manufacturing
Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available	Experimental flexibility
Reliable: nearly 300 references	Successfully used in many fields of research

Streptavidin is a 53kDa protein purified from Streptomyces avidinii, which has widespread applications due to its very high affinity to the vitamin biotin. Streptavidin is a tetrameric molecule, composed of 4 13kDa monomers, each of which can bind a molecule of biotin.

The Lightning-Link Streptavidin kit is unique in the sense that it is the only DIY streptavidin labeling kit available on the market. Like our biotinylation kits, Lightning-Link Streptavidin has been optimised to create high quality conjugates and minimise assay background. There is also no need for a wash or desalting step. The way in which the kits are optimised means the antibody or protein of interest will be labeled with up to 3 streptavidin molecules each, maximising the opportunity for biotin to bind and create a biotin streptavidin complex.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit is supplied with 3 vials, each suitable for labeling up to 10 ug of antibody.

Kit Contents for Lightning-Link (R) Streptavidin Antibody Labeling Kit

1 or 3 glass vial(s) of Lightning-Link mix
1 vial of LL-Modifier reagent
1 vial of LL-Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) Streptavidin

Easy Streptavidinlabeling of your antibody with 30 seconds hands-on time. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps. Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

Additional Lightning-Link (R) Streptavidin Products

Product Documents for Lightning-Link (R) Streptavidin Antibody Labeling Kit

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Manual - Lightning-Link (R) Streptavidin Antibody Labeling Kit

COA

SDS

Download SDS

Product Specific Notices for Lightning-Link (R) Streptavidin Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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