Human 17-Beta-Estradiol ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # KA0297
Key Product Details
Sample Type & Volume Required Per Well
Saliva and Tissue culture media (100 uL)
Sensitivity
28.5 pg/mL
Assay Range
29.3 to 30000 pg/mL
Product Specifications
Assay Type
Competitive ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
Quality control test: Standard curve. 17 beta-Estradiol ELISA Kit is a competitive immunoassay for the quantitative determination of 17 beta-Estradiol in biological and environmental samples.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 8.60%
Inter-Assay Precision (Precision between assays) CV% < 6.20%
Recovery for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)
Recovery
Cell Culture Supernatant: 92.20%, Saliva: 104.80%
Scientific Data Images for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)
ELISA: 17-Beta-Estradiol ELISA Kit [Alkaline Phosphatase] [KA0297] - Standard curve
Kit Contents for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)
- 17 beta-Estradiol ELISA Antibody: A yellow solution of a rabbit polyclonal antibody to 17 beta-Estradiol.
- 17 beta-Estradiol ELISA Conjugate: A blue solution of alkaline phosphatase conjugated with 17 beta-Estradiol.
- 17 beta-Estradiol Standard: A solution of 300,000 pg/mL 17 beta-Estradiol.
- Assay Buffer 3: Tris buffered saline containing proteins and detergents and sodium azide as a preservative
- Goat anti-Rabbit IgG Microtiter Plate: A plate using break-apart strips coated with goat antibody specific to rabbit IgG.
- Plate Sealer
- pNpp Substrate: A solution of p-nitrophenyl phosphate in buffer. Ready to use.
- Steroid Displacement Reagent: A special formulated displacer to inhibit steroid binding to proteins.
- Stop Solution: A solution of trisodium phosphate in water. Keep tightly capped. Caution: Caustic
- Wash Buffer Concentrate: Tris buffered saline containing detergents.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: 17-Beta-Estradiol
Alternate Names
17 beta Oestradiol, 17-Beta-Estradiol, 17-beta-HSD
Additional 17-Beta-Estradiol Products
Product Documents for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)
Product Specific Notices for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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