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Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66366

Novus Biologicals, part of Bio-Techne
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NBP2-66366

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50-4000 pg/mL

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human 5-LO in samples. No significant cross-reactivity or interference between Human 5-LO and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.46%

Inter-Assay Precision (Precision between assays) CV% < 9.46%

Recovery for Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

Recovery

84-108%

Linearity

Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

Scientific Data Images for Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

ELISA: Human 5-Lipoxygenase ELISA Kit (Chemiluminescence) [NBP2-66366] -

ELISA: Human 5-Lipoxygenase ELISA Kit (Chemiluminescence) [NBP2-66366] - Standard Curve Reference

Kit Contents for Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 5-Lipoxygenase

5 Lipoxygenase encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Alternate Names

5Lipoxygenase, 5LPG, ALOX5, LOG5, LOX5A

Gene Symbol

ALOX5

Additional 5-Lipoxygenase Products

Product Documents for Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human 5-Lipoxygenase ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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