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Mouse 5-Lipoxygenase ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66368

Novus Biologicals, part of Bio-Techne
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NBP2-66368

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse 5-LO in samples. No significant cross-reactivity or interference between Mouse 5-LO and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.17%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Mouse 5-Lipoxygenase ELISA Kit (Colorimetric)

Recovery

90-107%

Linearity

Mouse 5-Lipoxygenase ELISA Kit (Colorimetric)

Scientific Data Images for Mouse 5-Lipoxygenase ELISA Kit (Colorimetric)

Mouse 5-Lipoxygenase ELISA Kit (Colorimetric)

ELISA: Mouse 5-Lipoxygenase ELISA Kit (Colorimetric) [NBP2-66368] -

ELISA: Mouse 5-Lipoxygenase ELISA Kit (Colorimetric) [NBP2-66368] - Standard Curve Reference

Kit Contents for Mouse 5-Lipoxygenase ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 5-Lipoxygenase

5 Lipoxygenase encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Alternate Names

5Lipoxygenase, 5LPG, ALOX5, LOG5, LOX5A

Gene Symbol

ALOX5

Additional 5-Lipoxygenase Products

Product Documents for Mouse 5-Lipoxygenase ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse 5-Lipoxygenase ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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