Human A33/GPA33 - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human A33/GPA33 - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-30759

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30759

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue Homogenates, Cell Lysates and other Biological Fluids (100 uL)

Sensitivity

0.238 ng/mL (example only; lot dependent)

Assay Range

0.625 - 40 ng/mL (example only; lot dependent)

View all A33 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human A33/GPA33 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human A33/GPA33 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-30759] -

ELISA: Human A33/GPA33 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-30759] - Standard Curve Reference

Kit Contents for Human A33/GPA33 - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: A33

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Gene Symbol