Human ADAM10 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66435

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63-1000 pg/mL

View all ADAM10 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human ADAM10 in samples. No significant cross reactivity or interference between Human ADAM10 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10.42%

Inter-Assay Precision (Precision between assays) CV% < 9.77%

Recovery for Human ADAM10 ELISA Kit (Chemiluminescence)

Recovery

91-107%

Linearity

Human ADAM10 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human ADAM10 ELISA Kit (Chemiluminescence)

Human ADAM10 ELISA Kit (Chemiluminescence)

ELISA: Human ADAM10 ELISA Kit (Chemiluminescence) [NBP2-66435] -

ELISA: Human ADAM10 ELISA Kit (Chemiluminescence) [NBP2-66435] - Standard Curve Reference

Kit Contents for Human ADAM10 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ADAM10

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Long Name

A Disintegrin and Metalloprotease-like Domain 10

Alternate Names

CD156c, Kuzbanian, MADM

Gene Symbol

ADAM10

Additional ADAM10 Products

Product Documents for Human ADAM10 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human ADAM10 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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