Human ADAMTS4 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66443

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63-1000 pg/mL

View all ADAMTS4 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human ADAMTS4 in samples. No significant cross-reactivity or interference between Human ADAMTS4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.47%

Inter-Assay Precision (Precision between assays) CV% < 10.8%

Recovery for Human ADAMTS4 ELISA Kit (Chemiluminescence)

Recovery

87-114%

Linearity

Human ADAMTS4 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human ADAMTS4 ELISA Kit (Chemiluminescence)

Human ADAMTS4 ELISA Kit (Chemiluminescence)

ELISA: Human ADAMTS4 ELISA Kit (Chemiluminescence) [NBP2-66443] -

ELISA: Human ADAMTS4 ELISA Kit (Chemiluminescence) [NBP2-66443] - Standard Curve Reference

Kit Contents for Human ADAMTS4 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ADAMTS4

ADAMTS4, also known as aggrecanase-1, is a Metallopeptidase M12B type protease that is responsible for degradation of the articular cartilage protein aggrecan and the brain extracellular matrix proteoglycan brevican. The ADAMTS4 protein contains a signal sequence, a propeptide domain, a catalytic domain, a disintegrin-like motif, and a C-terminal domain with a thrombospondin (TSP) type 1 motif. ADAMTS4 has been implicated in inflammatory joint diseases, and it also may be a factor in the progression of glioma and Alzheimer's disease. ADAMTS4 expression has been documented in normal human brain, cartilage, heart, lung, placenta, muscle, and synovium. ESTs have been isolated from human tissue libraries, including normal brain, lung, pancreas, and skeletal muscle and cancerous breast.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 4

Alternate Names

ADMP-1, Aggrecanase 1

Gene Symbol

ADAMTS4

Additional ADAMTS4 Products

Product Documents for Human ADAMTS4 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human ADAMTS4 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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