Human AE Binding Protein 1/ACLP ELISA Kit (Colorimetric)

Name: Human AE Binding Protein 1/ACLP ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-27188

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27188

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.126 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

View all ACLP ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human AE Binding Protein 1/ACLP ELISA Kit (Colorimetric)

ELISA: Human AE Binding Protein 1/ACLP ELISA Kit (Colorimetric) [NBP3-27188] -

ELISA: Human AE Binding Protein 1/ACLP ELISA Kit (Colorimetric) [NBP3-27188] - Standard Curve Reference

Kit Contents for Human AE Binding Protein 1/ACLP ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ACLP

The adipocyte enhancer binding protein 1 is a transcriptional repressor with carboxypeptidase (CP) activity. This protein binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. Adipocyte-enhancer binding protein 1 may play a role in differentiated vascular smooth muscle cells.

Long Name

Aortic Carboxypeptidase-like Protein

Alternate Names

AEBP1

Gene Symbol