Bovine alpha 2-Macroglobulin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77018

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.078 ug/mL

Assay Range

0.312 ug/mL - 20 ug/mL

View all alpha 2-Macroglobulin ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Bovine

Specificity

This assay has high sensitivity and excellent specificity for detection of Bovine alpha 2-Macroglobulin. No significant cross-reactivity or interference between Bovine alpha 2-Macroglobulin and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Recovery for Bovine alpha 2-Macroglobulin ELISA Kit (Colorimetric)

Recovery

82 - 99%

Scientific Data Images for Bovine alpha 2-Macroglobulin ELISA Kit (Colorimetric)

Bovine alpha 2-Macroglobulin ELISA Kit (Colorimetric)

Bovine alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP2-77018] -

Bovine alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP2-77018] - Standard Curve Reference

Kit Contents for Bovine alpha 2-Macroglobulin ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay Plate (12 x 8 coated Microwells)
  • HRP-Conjugate (100 x concentrate)
  • HRP-Conjugate Diluent
  • Instruction Manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha 2-Macroglobulin

Alpha 2 macroglobulin is a serum protein which is mainly synthesised in the liver. It functions as a broad range irreversible proteinase inhibitor that forms a "trap" around most proteases. Alpha 2 macroglobulin is is a tetrameric molecule, and with a molecular weight of 720 kDa, it so large a molecule that it tends to remain intravascular. Following conformational change, the protinase is deposited within a central cavity where it is still active but prevented from further contact with its substrate. Removal of the complex is mediated by the generation of recognition sites which react with receptors on a variety of cells including macrophages and liver cells. Alpha 2 macroglobulin is localized on the surface of peripheral blood lymphocytes.

Alternate Names

A2M, alpha 2Macroglobulin, CPAMD5

Gene Symbol

A2M

Additional alpha 2-Macroglobulin Products

Product Documents for Bovine alpha 2-Macroglobulin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Bovine alpha 2-Macroglobulin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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