Mouse alpha-Synuclein ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08174

Novus Biologicals, part of Bio-Techne
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NBP3-08174
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

View all alpha-Synuclein ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.32

Inter-Assay Precision (Precision between assays) %CV < 6.17

Recovery for Mouse alpha-Synuclein ELISA Kit (Colorimetric)

Recovery

83 - 105%

Linearity

Mouse alpha-Synuclein ELISA Kit (Colorimetric)

Scientific Data Images for Mouse alpha-Synuclein ELISA Kit (Colorimetric)

Mouse alpha-Synuclein ELISA Kit (Colorimetric)

ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] -

ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] - Image from verified customer review.
Mouse alpha-Synuclein ELISA Kit (Colorimetric)

ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] -

ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] - DRC. Image from verified customer review.
Mouse alpha-Synuclein ELISA Kit (Colorimetric)

ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] -

ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] - Standard Curve Reference

Kit Contents for Mouse alpha-Synuclein ELISA Kit (Colorimetric)

  • Micro ELISA Plate (Dismountable) - 1 EA, Reference Standard - 2 vials, Concentrated Biotinylated Detection Ab (100X) - 120 uL, Concentrated HRP Conjugate (100X) - 120 uL, Reference Standard & Sample Diluent - 20 mL, Biotinylated Detection Ab Diluent - 14

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha-Synuclein

Alpha-synuclein, a member of the synuclein family, is a protein that was first identified in 1988 whose name is derived from its localization to both the synapse and nucleus (1-3). Specifically, it is expressed primarily in the brain, including Lewy Bodies (1-6). Alpha-synuclein is encoded by the SNCA gene, located on chromosome 4p21, and is processed as a 140 amino acid (aa) protein with a theoretical molecular weight of 14 kDa (1,2,4). Structurally alpha-synuclein consists of a N-terminal binding domain (1-60 aa), a central domain core region called the non-amyloid-beta component (NAC) (61-95 aa), and a C-terminal domain (96-140 aa) (1-3). The N-terminal region contains aa repeats with a KTKEGV consensus sequence that gives the protein its alpha-helical structure that associates with lipid membranes (1-4). The hydrophobic NAC region is responsible for alpha-synuclein aggregation and fibril formation (1-4). The acidic C-terminal tail is largely unstructured but can be targeted for post-translational modifications (1-4). The function of alpha-synuclein is not entirely understood, but it is shown to have a role in suppression of apoptosis, acting as a molecular chaperone, regulating glucose, and modulating calmodulin activity (1,3).

A number of studies have revealed that alpha-synuclein aggregation is a hallmark feature in a number of neurodegenerative diseases, referred to as synucleinopathies (2-4). Alpha-synuclein protein aggregates are a large component of Lewy bodies that are present in Parkinson's disease (PD), Lewy body dementia (LBD), and multiple system atrophy (1-6). Research has shown phosphorylation of alpha-synuclein at Ser129 moves the protein from the nucleus to the cytoplasm and promotes fibril formation associated with synucleinopathies (1,2,5). Recent studies also suggest that alpha-synuclein accumulation can prevent mitochondrial import machinery causing mitochondrial dysfunction that is often observed in neurodegeneration (5). It is thought that preventing alpha-synuclein aggregation may prevent PD, thus alpha-synuclein is a target for many potential therapeutic interventions aimed at decreasing aggregate formation or increasing clearance (1,2,4-6).

References

1. Villar-Pique, A., Lopes da Fonseca, T., & Outeiro, T. F. (2016). Structure, function and toxicity of alpha-synuclein: the Bermuda triangle in synucleinopathies. Journal of neurochemistry. https://doi.org/10.1111/jnc.13249

2. Emamzadeh F. N. (2016). Alpha-synuclein structure, functions, and interactions. Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences. https://doi.org/10.4103/1735-1995.181989

3. Burre J. (2015). The Synaptic Function of alpha-Synuclein. Journal of Parkinson's disease. https://doi.org/10.3233/JPD-150642

4. Lashuel, H. A., Overk, C. R., Oueslati, A., & Masliah, E. (2013). The many faces of alpha-synuclein: from structure and toxicity to therapeutic target. Nature reviews. Neuroscience. https://doi.org/10.1038/nrn3406

5. Rocha, E. M., De Miranda, B., & Sanders, L. H. (2018). Alpha-synuclein: Pathology, mitochondrial dysfunction and neuroinflammation in Parkinson's disease. Neurobiology of disease. https://doi.org/10.1016/j.nbd.2017.04.004

6. O'Leary, E. I., & Lee, J. C. (2019). Interplay between alpha-synuclein amyloid formation and membrane structure. Biochimica et biophysica acta. Proteins and proteomics. https://doi.org/10.1016/j.bbapap.2018.09.012

Alternate Names

NACP, PARK1, PARK4, SNCA, Synuclein-alpha

Gene Symbol

SNCA

Additional alpha-Synuclein Products

Product Documents for Mouse alpha-Synuclein ELISA Kit (Colorimetric)

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Product Specific Notices for Mouse alpha-Synuclein ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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