Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77015

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

9.7 mU/mL

Assay Range

39 mU/mL - 2500 mU/mL

View all Aminopeptidase N/CD13 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse Aminopeptidase N/CD13. No significant cross-reactivity or interference between Mouse Aminopeptidase N/CD13 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Recovery for Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric)

Recovery

82 - 104%

Scientific Data Images for Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric)

Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric)

Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric) [NBP2-77015] -

Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric) [NBP2-77015] - Standard Curve Reference

Kit Contents for Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay Plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction Manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Aminopeptidase N/CD13

Alanine aminopeptidase (CD13) is expressed on the majority of peripheral blood monocytes and granulocytes. CD13 is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines. CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma. CD13 is not expressed on B cells, T cells, platelets or erythrocytes. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. CD13 plays a role in biologically active peptide metabolism, in the control of growth and differentiation, in phagocytosis and in bactericidal/tumoricidal activities. CD13 also serves as a receptor for human coronaviruses.

CD13 is commonly used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. Therefore, CD13 antibodies are useful tools for cancer and renal disorder research.

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Gene Symbol

ANPEP

Additional Aminopeptidase N/CD13 Products

Product Documents for Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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