Porcine Angiopoietin-1 ELISA Kit (Colorimetric)

Name: Porcine Angiopoietin-1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-77020

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77020

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

6 ng/mL

Assay Range

6 ng/mL - 200 ng/mL

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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Porcine

Specificity

This assay has high sensitivity and excellent specificity for detection of porcine Angiopoietin-1. No significant cross-reactivity or interference between porcine Angiopoietin-1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Recovery for Porcine Angiopoietin-1 ELISA Kit (Colorimetric)

Recovery

90 - 105%

Scientific Data Images for Porcine Angiopoietin-1 ELISA Kit (Colorimetric)

Porcine Angiopoietin-1 ELISA Kit (Colorimetric) [NBP2-77020] -

Porcine Angiopoietin-1 ELISA Kit (Colorimetric) [NBP2-77020] - Standard Curve Reference

Kit Contents for Porcine Angiopoietin-1 ELISA Kit (Colorimetric)

Adhesive Strip (For 96 wells)
Antibody
Assay Plate
HRP-Conjugate
Instruction Manual
Standard
Stop Solution
Substrate A
Substrate B
Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Angiopoietin-1

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Alternate Names

ANGPT1

Gene Symbol