Human Annexin V ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91253
Key Product Details
Sample Type & Volume Required Per Well
Cell culture supernatant, serum (50 uL)
Sensitivity
0.33 ng/mL
Assay Range
0.78 - 50 ng/mL
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human Annexin V positive serum. There was no crossreactivity detected, notably not with Annexin VIII (Vac beta).
Description
The human Annexin V ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Annexin V.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 10%
Inter-Assay Precision (Precision between assays) CV% < 11.00%
Recovery for Human Annexin V ELISA Kit (Colorimetric)
Recovery
The overall mean recovery was 85%. Please see protocol for more specific Spike Recovery details.
Scientific Data Images for Human Annexin V ELISA Kit (Colorimetric)
![ELISA: Human Annexin V ELISA Kit (Colorimetric) [NBP1-91253]](https://resources.bio-techne.com/images/products/Annexin-V-ELISA-Kit-ELISA-NBP1-91253-img0001.jpg "ELISA: Human Annexin V ELISA Kit (Colorimetric) [NBP1-91253]")
ELISA: Human Annexin V ELISA Kit (Colorimetric) [NBP1-91253]
ELISA: Annexin V ELISA Kit [NBP1-91253] - Standard curveKit Contents for Human Annexin V ELISA Kit (Colorimetric)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Annexin V
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human Annexin V monoclonal antibody
- Human Annexin V Standard lyophilized, 100 ng/ml upon reconstitution
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Annexin V
Alternate Names
ANX5, ANXA5, ANXV
Gene Symbol
ANXA5
Additional Annexin V Products
Product Documents for Human Annexin V ELISA Kit (Colorimetric)
Product Specific Notices for Human Annexin V ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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