Apolipoprotein A-I/ApoA1 Antibody Pair [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79506

Novus Biologicals, part of Bio-Techne
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Key Product Details

Assay Type

Sandwich ELISA

Assay Range

250-8000 pg/ml (example only; lot dependent)

Sensitivity

250 pg/ml (example only; lot dependent)

Reactivity

Human

View all Apolipoprotein A-I/ApoA1 ELISA Kits »

Product Specifications

Description

Solid Phase sandwich ELISA for the quantitative determination of Human Apolipoprotein A-I/ApoA1.

Sample Volume Required

100 ul

Conjugate

HRP

Scientific Data Images for Apolipoprotein A-I/ApoA1 Antibody Pair [HRP]

Sandwich ELISA: Apolipoprotein A-I/ApoA1 Antibody Pair [HRP] [NBP2-79506] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for Apolipoprotein A-I/ApoA1 Antibody Pair [HRP]

  • Rabbit Monoclonal Capture Antibody: (Catalog # NBP2-89599)
  • Rabbit Polyclonal Detection Antibody (HRP-conjugated)
  • Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Apolipoprotein A-I/ApoA1 Antibody Pair [HRP]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein A-I/ApoA1 Antibody Pair [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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