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Rat ARNT2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69862

Novus Biologicals, part of Bio-Techne
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NBP2-69862

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat ARNT2 in samples. No significant cross-reactivity or interference between Rat ARNT2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.67%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Rat ARNT2 ELISA Kit (Colorimetric)

Recovery

88-108%

Linearity

Rat ARNT2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat ARNT2 ELISA Kit (Colorimetric)

Rat ARNT2 ELISA Kit (Colorimetric)

ELISA: Rat ARNT2 ELISA Kit (Colorimetric) [NBP2-69862] -

ELISA: Rat ARNT2 ELISA Kit (Colorimetric) [NBP2-69862] - Standard Curve Reference

Kit Contents for Rat ARNT2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ARNT2

AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common "PAS" motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. BMAL1 (Brain and Muscle Arnt-like protein 1), also designated Arnt 3, TIC, JAP3 or MOP-3, has been shown to dimerize with Clock and bind to the promoter region of mPer1, suggesting that this protein plays a role in regulation of circadian oscillation in mammals.

Alternate Names

ARNT protein 2, aryl-hydrocarbon receptor nuclear translocator 2, bHLHe1BHLHE1, Class E basic helix-loop-helix protein 1, KIAA0307aryl hydrocarbon receptor nuclear translocator 2

Gene Symbol

ARNT2

Additional ARNT2 Products

Product Documents for Rat ARNT2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat ARNT2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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