BAFF/BLyS/TNFSF13B Antibody Pair [Biotin]

Sandwich ELISA

16 - 1000 pg/ml

Cell Culture Supernatant, Plasma, Serum (100 ul)

16 pg/ml

Human

Sandwich ELISA for the quantitative determination of Human BAFF/BLyS/TNFSF13B

100 ul

Biotin

1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (rat monoclonal antibody) to 2ug/ml in PBS without carrier protein and use it fresh.
2. Dilute the desired amount of Detection Antibody (DET) (1 mg/ml) (biotinylated rat monoclonal antibody) to 1 ug/ml in Blocking Buffer and use it fresh.
3. Reconstitute the Streptavidin-HRP (STREP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted STREP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
4. Reconstitute the Standard Protein (STD) (recombinant human BAFF/BLyS/TNFSF13B) with 100 ul ELISA Buffer to obtain a concentration of 50 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 1000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.25 pg/ml, 15.6 pg/ml and 0 pg/ml.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.