Rat beta-NGF ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0401

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Serum (100 uL)

Sensitivity

<1 pg/mL

Assay Range

15.6 to 1000 pg/mL

View all beta-NGF ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Description

Ngf (Rat) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of rat Ngf.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.4%

Inter-Assay Precision (Precision between assays) CV% < 6.1%

Kit Contents for Rat beta-NGF ELISA Kit (Colorimetric)

  • 96-well plate precoated with anti- rat NGF antibody
  • ABC diluent buffer
  • Adhesive cover
  • Antibody diluent buffer
  • Avidin-Biotin-Peroxidase Complex (ABC) (dilution 1:100)
  • Biotinylated anti- rat NGF antibody (dilution 1:100)
  • Lyophilized recombinant rat NGF standard
  • Sample diluent buffer
  • TMB color developing agent
  • TMB stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-NGF

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.

Long Name

beta Nerve Growth Factor

Alternate Names

betaNGF, NGFB

Gene Symbol

NGF

Additional beta-NGF Products

Product Documents for Rat beta-NGF ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat beta-NGF ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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