Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70057

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

View all Carbonic Anhydrase III/CA3 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human CA3 in samples. No significant cross-reactivity or interference between Human CA3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.97%

Inter-Assay Precision (Precision between assays) CV% < 6.03%

Recovery for Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

Recovery

87-106%

Linearity

Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

Scientific Data Images for Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

ELISA: Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) [NBP2-70057] -

ELISA: Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric) [NBP2-70057] - Standard Curve Reference

Kit Contents for Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Carbonic Anhydrase III/CA3

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.

Alternate Names

CA3

Gene Symbol

CA3

Additional Carbonic Anhydrase III/CA3 Products

Product Documents for Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Carbonic Anhydrase III/CA3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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