Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70059

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all Carbonic Anhydrase VB/CA5B ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human CA5B in samples. No significant cross-reactivity or interference between Human CA5B and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.28%

Inter-Assay Precision (Precision between assays) CV% < 4.84%

Recovery for Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

Recovery

86-104%

Linearity

Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

Scientific Data Images for Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

ELISA: Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric) [NBP2-70059] -

ELISA: Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric) [NBP2-70059] - Standard Curve Reference

Kit Contents for Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Carbonic Anhydrase VB/CA5B

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes responsible for catalyzing the reversible hydration of carbon dioxide.CAs show extensive diversity in their distribution and subcellular localization.They are involved in a variety of biological processes, including calcification, bone resorption, respiration, acid-base balance and the formation of aqueous humor, saliva, gastric juice and cerebrospinal fluid. CA VB, also known as carbonate dehydratase VB, is one of two isoforms of CA V. It localizes to the mitochondria and is involved in metabolic processes. CA VB is predominantly expressed in heart, pancreas, lung, placenta, kidney and skeletal muscle. It exhibits highest homology with family member CA VA (the second isoform of CA V); however, unlike CA VA, it is not expressed in the liver, suggesting that it plays a significantly different physiological role.

Alternate Names

CA5B, CAVB

Gene Symbol

CA5B

Additional Carbonic Anhydrase VB/CA5B Products

Product Documents for Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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