Human Cathepsin F ELISA Kit (Colorimetric)

Name: Human Cathepsin F ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-18705

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18705

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Product Specifications
Precision
Recovery
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture, Tissue Extract

Sensitivity

0.5 ng/mL (example only; lot dependent)

Assay Range

0.781 - 50 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Precision

Intra-Assay Precision (Precision within an assay) CV%< 6.3 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) CV%< 10.3 (example only; lot dependent)

Recovery for Human Cathepsin F ELISA Kit (Colorimetric)

Recovery

98% (example only; lot dependent)

Scientific Data Images for Human Cathepsin F ELISA Kit (Colorimetric)

ELISA: Human Cathepsin F ELISA Kit (Colorimetric) [NBP3-18705]

ELISA: Human Cathepsin F ELISA Kit (Colorimetric) [NBP3-18705] - Standard Reference Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: Cathepsin F

Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W.

Alternate Names

CTSF

Gene Symbol