Human Cathepsin L ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91259

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

1.7 ng/mL

Assay Range

3.1 - 50 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Cathepsin L positive serum. No crossreactivity was detected, namely not with Cathepsin V (80% aa homology).

Description

The human Cathepsin L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Cathepsin L

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.00%

Inter-Assay Precision (Precision between assays) CV% < 6.20%

Scientific Data Images for Human Cathepsin L ELISA Kit (Colorimetric)

Human Cathepsin L ELISA Kit [NBP1-91259] - Data for Human Cathepsin L Platinum ELISA

Kit Contents for Human Cathepsin L ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Cathepsin L
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human Cathepsin L monoclonal antibody
  • Human Cathepsin L Standard lyophilized, 100 ng/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cathepsin L

Cathepsin L is closely related to the recently described cathepsin V (L2) and the sequence indentity of almost 80% between them requires antibodies for the detection of cathepsin L which do not crossreact with cathepsin V. This requirement can only be met by epitope specific monoclonal antibodies as are used in this antibody. Cathepsin L is a lysosomal cysteine proteinase consisting of a heavy chain of about 25 kDa and a light chain of about 5 kDa derived proteolytically from the same precursor. Cathepsin L has been shown to be produced by many different cell types. Since cathepsin L is capable of degrading protein constituents of the extracellular matrix it is assumed to play a crucial role in tumor progression and metastasis and a number of other disorders where the destruction of the extracellular matrix is the major cause of disease (e.g. rheumatoid arthritis, neurodegeneration).

Alternate Names

CTSL, CTSL1

Gene Symbol

CTSL

Additional Cathepsin L Products

Product Documents for Human Cathepsin L ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Cathepsin L ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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