Human CCL2/MCP1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91276

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid (20 uL)

Sensitivity

2.3 pg/mL

Assay Range

15.6 - 1,000 pg/mL

View all CCL2/MCP1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human MCP-1. The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human MCP- 1 positive serum. There was no cross reactivity detected, namely not with MCP-3.

Description

The human MCP-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human MCP-1.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.70%

Inter-Assay Precision (Precision between assays) CV% < 8.70%

Recovery for Human CCL2/MCP1 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 84% to 98% with an overall mean recovery of 92%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human CCL2/MCP1 ELISA Kit (Colorimetric)

ELISA: Human CCL2/MCP1 ELISA Kit (Colorimetric) [NBP1-91276]

ELISA: Human CCL2/MCP1 ELISA Kit (Colorimetric) [NBP1-91276]

ELISA: CCL2/MCP1 ELISA Kit [NBP1-91276] - Standard curve

Kit Contents for Human CCL2/MCP1 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a human MCP-1 Standard curve (coloured)
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human MCP-1, HRP-Conjugate (human MCP-1 monoclonal antibody) and Assay Buffer, lyophilized
  • Assay Buffer Concentrate 20x (Use when an external predilution of the samples is needed)
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CCL2/MCP1

MCP-1was characterized as a monocyte-specific chemoattractant that was later shown to attract also T lymphocytes and NK cells. The human mature MCP-1 protein consists of 76 amino acids, derived by cleavage of a hydrophobic signal peptide from the 99 aa precursor protein. MCP-1 is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF-alpha and IL-1beta, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells. Because of its target cell specificity, MCP-1 was postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally it has been shown to chemoattract CD4+ and CD8+ T lymphocytes, and expression of the MCP-1 chemokine may affect HIV infection via signaling through the CCR2 receptor.

Alternate Names

C-C motif chemokine 2, chemokine (C-C motif) ligand 2, GDCF-2, HC11Monocyte secretory protein JE, MCAFMonocyte chemotactic and activating factor, MCP1 Monocyte chemotactic protein 1, MCP-1 Small-inducible cytokine A2, MGC9434, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, SCYA2HSMCR30, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, SMC-CF

Gene Symbol

CCL2

Additional CCL2/MCP1 Products

Product Documents for Human CCL2/MCP1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human CCL2/MCP1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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