Mouse CCL2/MCP1 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92659
Key Product Details
Sample Type & Volume Required Per Well
Cell culture supernatant, serum (50 uL)
Sensitivity
2.2 pg/mL
Assay Range
15.6 - 1,000 pg/mL
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Mouse
Specificity
The assay detects both natural and recombinant mouse MCP-1. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse MCP-1 positive serum. There was no crossreactivity detected, notably not with TCA3 and MIP-1 beta.
Description
The mouse MCP-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse MCP-1
Precision
Intra-Assay Precision (Precision within an assay) CV% < 6.20%
Inter-Assay Precision (Precision between assays) CV% < 5.60%
Recovery for Mouse CCL2/MCP1 ELISA Kit (Colorimetric)
Recovery
The recovery ranged from 80% to 111% with an overall mean recovery of 98%. Please see protocol for more specific Spike Recovery details.
Scientific Data Images for Mouse CCL2/MCP1 ELISA Kit (Colorimetric)
Mouse CCL2/MCP1 ELISA Kit [NBP1-92659] - Data for Mouse MCP-1 Platinum ELISA
Kit Contents for Mouse CCL2/MCP1 ELISA Kit (Colorimetric)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse MCP-1
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-mouse MCP-1 monoclonal antibody
- Mouse MCP-1 Standard lyophilized, 2 ng/ml upon reconstitution
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: CCL2/MCP1
Alternate Names
C-C motif chemokine 2, chemokine (C-C motif) ligand 2, GDCF-2, HC11Monocyte secretory protein JE, MCAFMonocyte chemotactic and activating factor, MCP1 Monocyte chemotactic protein 1, MCP-1 Small-inducible cytokine A2, MGC9434, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, SCYA2HSMCR30, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, SMC-CF
Gene Symbol
CCL2
Additional CCL2/MCP1 Products
Product Documents for Mouse CCL2/MCP1 ELISA Kit (Colorimetric)
Product Specific Notices for Mouse CCL2/MCP1 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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