Human CCL8/MCP-2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68059

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

3.75 pg/mL

Assay Range

6.25 - 400 pg/mL

View all CCL8/MCP-2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human MCP-2/CCL8 in samples. No significant cross-reactivity or interference between Human MCP-2/CCL8 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.36%

Inter-Assay Precision (Precision between assays) CV% < 5.57%

Recovery for Human CCL8/MCP-2 ELISA Kit (Colorimetric)

Recovery

84-109%

Linearity

Human CCL8/MCP-2 ELISA Kit (Colorimetric)

Scientific Data Images for Human CCL8/MCP-2 ELISA Kit (Colorimetric)

Human CCL8/MCP-2 ELISA Kit (Colorimetric)

ELISA: Human CCL8/MCP-2 ELISA Kit (Colorimetric) [NBP2-68059] -

ELISA: Human CCL8/MCP-2 ELISA Kit (Colorimetric) [NBP2-68059] - Standard Curve Reference

Kit Contents for Human CCL8/MCP-2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CCL8/MCP-2

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MCP-2, MCP2

Gene Symbol

CCL8

Additional CCL8/MCP-2 Products

Product Documents for Human CCL8/MCP-2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CCL8/MCP-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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