Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92662

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

0.14 ng/mL

Assay Range

0.31 - 20.0 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse CD40L positive serum. There was no crossreactivity detected.

Description

The mouse sCD40L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse sCD40L

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.50%

Inter-Assay Precision (Precision between assays) CV% < 11.10%

Recovery for Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Recovery

The overall mean recovery was 85%. Recoveries were shown to depend on the serum used. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Mouse CD40 Ligand/TNFSF5 ELISA Kit [NBP1-92662] - Data for Mouse sCD40L Platinum ELISA

Kit Contents for Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse sCD40L
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse sCD40L monoclonal antibody
  • Mouse sCD40L Standard lyophilized, 40 ng/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD40 Ligand/TNFSF5

The murine cell surface antigen is known also as Ly62. CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.) A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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