Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75152

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all CD40 Ligand/TNFSF5 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CD40L in samples. No significant cross-reactivity or interference between Rat CD40L and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.8%

Inter-Assay Precision (Precision between assays) CV% < 4.41%

Recovery for Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Recovery

89-110%

Linearity

Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Scientific Data Images for Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

ELISA: Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) [NBP2-75152] -

ELISA: Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) [NBP2-75152] - Standard Curve Reference

Kit Contents for Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD40 Ligand/TNFSF5

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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