Human CD81 ELISA Kit (Colorimetric)

Name: Human CD81 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-18222

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18222

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and cell culture supernates (100 ul)

Sensitivity

0.112 ng/ml

Assay Range

0.312-20 ng/ml

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human CD81 in the sample, this Human CD81 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human CD81 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 15

Recovery for Human CD81 ELISA Kit (Colorimetric)

Recovery

81-117%

Scientific Data Images for Human CD81 ELISA Kit (Colorimetric)

ELISA: Human CD81 ELISA Kit (Colorimetric) [NBP3-18222]

ELISA: Human CD81 ELISA Kit (Colorimetric) [NBP3-18222] - Standard Curve Reference

Kit Contents for Human CD81 ELISA Kit (Colorimetric)

Balance Solution
Enzyme Conjugate
Microtiter Plate
Standards
Stop Solution
Substrate A
Substrate B
Wash Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD81

CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse. (1) CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors. (2) Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha. (2) The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro. (2)

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

Additional CD81 Products

Product Documents for Human CD81 ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Manual - Human CD81 ELISA Kit (Colorimetric)

COA

SDS

Download SDS

Product Specific Notices for Human CD81 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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