Human CD97 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20177

Novus Biologicals, part of Bio-Techne
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NBP3-20177
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Key Product Details

Sample Type & Volume Required Per Well

Serum (100 ul)

Sensitivity

15.80 pg/mL

Assay Range

23.44-1500 pg/mL (example only; lot dependent)

View all CD97 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Human

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.3

Inter-Assay Precision (Precision between assays) CV% < 10.7

Recovery for Human CD97 ELISA Kit (Colorimetric)

Recovery

85-87%

Kit Contents for Human CD97 ELISA Kit (Colorimetric)

  • 96 well microplate coated with capture antibody
  • Color Reagent A
  • Color Reagent B
  • Detection Antibody conjugated to HRP
  • Dilution Buffer Concentration
  • Sample Dilution Buffer Concentrate
  • Standards
  • Stop Solution
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD97

CD97, a CD55 Receptor, is a leukocyte antigen located on most activated leukocytes. CD97 is highly homologous to EMR2 with five EGF-like domains in the N-terminal extracellular domain, suggesting that it may be involved in cell signaling as well as in cell adhesion (Hamann et al., 1996). Two variants are produced by alternative splicing, where the shorter form lacks exons 5 and 6 and reduces the number of EGF-like domains from 5 to 3, compared to the full-length isoform. CD97 is associated with with the activated lesions of multiple sclerosis. CD97 expression is also implicated in the dedifferentiation of colon and thyroid tumors. CD97 is expressed abundantly in cells of hematopoietic origin and is markedly upregulated on activated T and B cells. ESTs have been isolated from a wide variety of tissue libraries.

Alternate Names

CD97

Gene Symbol

ADGRE5

Additional CD97 Products

Product Documents for Human CD97 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CD97 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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