Human CDC2/CDK1 ELISA Kit (Colorimetric)

Name: Human CDC2/CDK1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-75169

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75169

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all CDC2/CDK1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human CDK1 in samples. No significant cross-reactivity or interference between Human CDK1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.24%

Inter-Assay Precision (Precision between assays) CV% < 5.79%

Recovery for Human CDC2/CDK1 ELISA Kit (Colorimetric)

Recovery

85-108%

Linearity

Scientific Data Images for Human CDC2/CDK1 ELISA Kit (Colorimetric)

ELISA: Human CDC2/CDK1 ELISA Kit (Colorimetric) [NBP2-75169] -

ELISA: Human CDC2/CDK1 ELISA Kit (Colorimetric) [NBP2-75169] - Standard Curve Reference

Kit Contents for Human CDC2/CDK1 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CDC2/CDK1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Gene Symbol