Rat CENPB ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75227

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all CENPB ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CENPB in samples. No significant cross-reactivity or interference between Rat CENPB and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.81%

Inter-Assay Precision (Precision between assays) CV% < 4.17%

Recovery for Rat CENPB ELISA Kit (Colorimetric)

Recovery

86-108%

Linearity

Rat CENPB ELISA Kit (Colorimetric)

Scientific Data Images for Rat CENPB ELISA Kit (Colorimetric)

Rat CENPB ELISA Kit (Colorimetric)

ELISA: Rat CENPB ELISA Kit (Colorimetric) [NBP2-75227] -

ELISA: Rat CENPB ELISA Kit (Colorimetric) [NBP2-75227] - Standard Curve Reference

Kit Contents for Rat CENPB ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CENPB

CENPB product is a highly conserved protein associated with the centromere. It is a DNA-binding protein containing a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Alternate Names

CENP-B, centromere autoantigen B, Centromere protein B, centromere protein B (80kD), centromere protein B, 80kDa, major centromere autoantigen B

Gene Symbol

CENPB

Additional CENPB Products

Product Documents for Rat CENPB ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CENPB ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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