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Rat CHD5 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75271

Novus Biologicals, part of Bio-Techne
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NBP2-75271

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CHD5 in samples. No significant cross-reactivity or interference between Rat CHD5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.13%

Inter-Assay Precision (Precision between assays) CV% < 5.24%

Recovery for Rat CHD5 ELISA Kit (Colorimetric)

Recovery

87-105%

Linearity

Rat CHD5 ELISA Kit (Colorimetric)

Scientific Data Images for Rat CHD5 ELISA Kit (Colorimetric)

Rat CHD5 ELISA Kit (Colorimetric)

ELISA: Rat CHD5 ELISA Kit (Colorimetric) [NBP2-75271] -

ELISA: Rat CHD5 ELISA Kit (Colorimetric) [NBP2-75271] - Standard Curve Reference

Kit Contents for Rat CHD5 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CHD5

Changes in gene expression are associated with changes in chromatin structure, which can result from modification of histones by acetylation and methylation. In addition, SWI/SNF proteins can effect chromatin structure and gene expression by causing ATP-dependent disruption of the nucleosome and by enhancing binding of transcription factors. CHD5 belongs to a group of SWI/SNF proteins called CHD proteins, which contain a SWI/SNF-like helicase/ATPase domain, as well as a DNA-binding domain and a chromodomain that directly modifies chromatin structure (Thompson et al., 2003 (PubMed 12592387)).(supplied by OMIM)

Alternate Names

ATP-dependent helicase CHD5, CHD-5, chromodomain helicase DNA binding protein 5, chromodomain-helicase-DNA-binding protein 5, DKFZp434N231, EC 3.6.1, EC 3.6.4.12, KIAA0444

Gene Symbol

CHD5

Additional CHD5 Products

Product Documents for Rat CHD5 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CHD5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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