Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75274

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

View all Chitotriosidase/CHIT1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human CHIT1 in samples. No significant cross-reactivity or interference between Human CHIT1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.04%

Inter-Assay Precision (Precision between assays) CV% < 4.97%

Recovery for Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

Recovery

84-106%

Linearity

Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

Scientific Data Images for Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

ELISA: Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric) [NBP2-75274] -

ELISA: Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric) [NBP2-75274] - Standard Curve Reference

Kit Contents for Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Chitotriosidase/CHIT1

Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. [provided by RefSeq]

Alternate Names

CHI3, CHIT1

Gene Symbol

CHIT1

Additional Chitotriosidase/CHIT1 Products

Product Documents for Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Chitotriosidase/CHIT1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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