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Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75264

Novus Biologicals, part of Bio-Techne
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NBP2-75264

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human CHAc in samples. No significant cross-reactivity or interference between Human CHAc and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.36%

Inter-Assay Precision (Precision between assays) CV% < 5.62%

Recovery for Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

Recovery

88-104%

Linearity

Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

Scientific Data Images for Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

ELISA: Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric) [NBP2-75264] -

ELISA: Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric) [NBP2-75264] - Standard Curve Reference

Kit Contents for Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Choline Acetyltransferase/ChAT

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Alternate Names

CHAT, Choline acetylase

Gene Symbol

CHAT

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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