Human Chorionic Gonadotropin alpha Chain (hCG alpha) ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80329

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum (100 ul)

Sensitivity

3.4 pg/ml

Assay Range

15.63-1000 pg/ml (example only; lot dependent)

View all Chorionic Gonadotropin alpha Chain (HCG alpha) ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Human

Specificity

Recognizes both recombinant and natural Human Chorionic Gonadotropin alpha Chain (hCG alpha).

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.67

Inter-Assay Precision (Precision between assays) CV% < 4.9

Recovery

Recovery

serum (n=3): 81 -87%
urine (n=3): 81 -87%

Scientific Data Images

ELISA: Human Chorionic Gonadotropin alpha Chain (hCG alpha) ELISA Kit (Colorimetric) [NBP2-80329]

ELISA: Human Chorionic Gonadotropin alpha Chain (hCG alpha) ELISA Kit (Colorimetric) [NBP2-80329]

ELISA: Human Chorionic Gonadotropin alpha Chain (hCG alpha) ELISA Kit (Colorimetric) [NBP2-80329] - Example standard curve for Human Chorionic Gonadotropin alpha Chain (hCG alpha) ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents

  • 1. 96 well microplate coated with Capture Antibody
  • 2. Detection Antibody conjugated to HRP
  • 3. Standards
  • 4. Wash Buffer Concentrate
  • 5. Dilution Buffer Concentrate
  • 6. Color Reagent A
  • 7. Color Reagent B
  • 8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: Chorionic Gonadotropin alpha Chain (HCG alpha)

HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.

Alternate Names

alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA

Gene Symbol

CGA

Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products

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Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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