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Human, Monkey Coagulation Factor XIV/Protein C ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1840

Novus Biologicals, part of Bio-Techne
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KA1840

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Samples, Milk, Saliva and Urine (50 uL)

Sensitivity

2.5 ng/mL

Assay Range

3.125 to 200 ng/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human, Monkey

Description

Quality control test: Standard curve. PROC (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human PROC.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.8%

Inter-Assay Precision (Precision between assays) CV% < 7.3%

Recovery

Recovery

98%

Scientific Data Images

ELISA: Human, Monkey Coagulation Factor XIV/Protein C ELISA Kit (Colorimetric) [KA1840]

ELISA: Human, Monkey Coagulation Factor XIV/Protein C ELISA Kit (Colorimetric) [KA1840]

ELISA: Coagulation Factor XIV/Protein C ELISA Kit [Biotin] [KA1840] - Standard curve

Kit Contents

  • Biotinylated Protein C Antibody (100x): A 100-fold concentrated biotinylated polyclonal antibody against Protein C.
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine.
  • Human Protein C Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human Protein C.
  • Human Protein C Standard: Human Protein C in a buffered protein base (lyophilized).
  • Sealing Tapes: Precut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
  • Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  • Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Coagulation Factor XIV/Protein C

Protein C is a vitamin K-dependent serine protease that is produced in the liver and made up of 2 polypeptide chains. The 62 kDa proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa inhibiting blood coagulation. The molecular weight of the active enzyme is 55 kDa and the normal concentration in human plasma is approximately 1-3 ng/ml while the concentration of the proenzyme is approximately 3 ug/mL due to the very fast turnover.

Long Name

Vitamin K-dependent Protein C

Alternate Names

Autoprothrombin IIA, PROC, Protein C

Gene Symbol

PROC

Additional Coagulation Factor XIV/Protein C Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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